This page provides a list of two-photon probes as well as a matching wavelength range that has been already used,
and the reference where the two-photon absorption spectrum can be found.
Number of two-photon probes:
Name | Wavelength(s) for two-photon excitation | Reference or URL | Available spectrum | Comments |
---|---|---|---|---|
Acridine Orange | 837 > 882 >> 981 | Bestvater 2002 | Figure 3 | Nucleic acids |
Alexa Fluor 430 | 910 (880-940) | Mütze 2012 | Figure 2 | |
Alexa Fluor 488 | 720-800, 900-1000 | Mütze 2012 | Figure 2 | |
Alexa Fluor 514 | 750-800, 950-1000 | Mütze 2012 | Figure 2 | |
Alexa Fluor 546 | 720-840, 950-1000 | Mütze 2012 | Figure 2 | |
Alexa Fluor 568 | 720-840 | Mütze 2012 | Figure 2 | |
Alexa Fluor 594 | 720-850 | Mütze 2012 | Figure 2 | |
Alexa Fluor 610 | 720-900 | Mütze 2012 | Figure 2 | |
Alexa Fluor 633 | 720-900 | Mütze 2012 | Figure 2 | Artery labelling (see Shen 2012) |
Alexa Fluor 647 | 1240 | Kobat 2009 | Figure 7 (far-red) | OPO-friendly |
Alexa Fluor 680 | 1280 | Kobat 2009 | Figure 7 (far-red) | OPO-friendly |
Alexa Fluor 700 | 1300 | Kobat 2009 | Figure 7 (far-red) | OPO-friendly |
Aminomethylcoumarin (AMC) | 703 < 722 >> 863 | Bestvater 2002 | Figure 3 | |
Aminomethyl coumarin acetate (AMCA) | 700 | Bok 2015 | no | |
Ant2-PHEA | 800 | Mettra 2016 | Figure 6 | Endothelium staining |
ArcLight A242 | 950 | Brinks 2015 | Table 1 | Voltage-sensitive |
ASAP1 | 950 | Brinks 2015 | Table 1 | Voltage-sensitive |
Bodipy (in water) | 920 | DRBIO, Cornell University | Online | Calcium indicator |
Bodipy FL (in DMSO) | 920 << 972 | Bestvater 2002 | Figure 3 | |
Bodipy TRoad | 1032 << 1108 | Bestvater 2002 | Figure 3 | |
Brilliant Violet 421 (BV421) | 710-1000 | Chattopadhyay 2012 | no (cf. Fig 6G) | |
CAESR | 968 | Brinks 2015 | Table 1 | Voltage-sensitive |
Cal-590 | 1050 | Tischbirek 2015 | Figure 1B | Calcium indicator |
Calcium Crimson | 850-920 | DRBIO, Cornell University | Online | Calcium indicator |
Calcium Green | 950 > 820 | DRBIO, Cornell University | Online | Calcium indicator |
Calcium Orange | 820 | DRBIO, Cornell University | Online | Calcium indicator |
Calcofluor White | 590 | Trägårdh 2015 | Figure 5 | Cellulose and chitin binding |
CaRuby-Cl | 912 | Collot 2012 | Figure 5 | Calcium indicator |
CaRuby-F | 917 | Collot 2012 | Figure 5 | Calcium indicator |
CaRuby-Me | 917 | Collot 2012 | Figure 5 | Calcium indicator |
Cascade Blue | 700-800 | DRBIO, Cornell University | Online | ! Poorly efficient |
CellTracker Blue (CMAC) | 780 | Zinselmeyer 2009 | no | |
CellTracker Orange (CMTMR) | 820 | Miller 2002 | no | |
CFP | 840 | Zipfel 2003 | Figure 3b | |
CFSE | 780 | Miller 2002 | no | |
Citrine | 968 | Drobizhev 2011 | Figure 1 | |
Coumarin 307 | 800 | DRBIO, Cornell University | Online | |
Cy2 | 837 < 908 > 981 | Bestvater 2002 | Figure 4 | |
Cy3 | 1032 | Bestvater 2002 | Figure 4 | |
Cy5.5 | 1280 | Kobat 2009 | Figure 7 (far-red) | OPO-friendly |
DAPI | 580 >> 685 | Trägårdh 2015 | Figure 2 | Nucleic acids |
DCF2 (H2DCFDA) (in DMSO) | 1065 | Bestvater 2002 | no | |
DiA | 880 | Ruthazer 2002 | Table 1 | Lipophilic Tracer |
DiD | 830 | Ruthazer 2002 | Table 1 | Lipophilic Tracer |
DiI | 750-, 1000-1050 | DRBIO, Cornell University | Online | Lipophilic Tracer |
DiO | 880 | Ruthazer 2002 | Table 1 | Lipophilic Tracer |
Di-4-ANEPPS | 940 | DRBIO, Cornell University | Online | Voltage-sensitive |
Di-8-ANEPPDHQ | 900-950 | Fisher 2015 | Figure 17.3 | Voltage-sensitive |
Di-stryl benzene derivative: 2,5-Dicyano-1,4-bis[2-(4-(di-n-butylamino)phenyl)Vinyl]benzene |
830 | Pond 2002 | Figure 3(1), Table 2 | Hydrophobic |
Di-stryl benzene derivative: 2,5-Dicyano-1,4-bis[2-(4-(diphenylamino)phenyl)Vinyl]benzene |
830 | Pond 2002 | Figure 3(2), Table 2 | Hydrophobic |
Di-stryl benzene derivative: 1,4-Bis[1-cyano-2-(4-(di-n-butylamino)phenyl)Vinyl]benzene |
790 | Pond 2002 | Figure 3(3), Table 2 | Hydrophobic |
Di-stryl benzene derivative: 1,4-Bis[1-cyano-2-(4-(diphenylamino)phenyl)Vinyl]benzene |
825 | Pond 2002 | Figure 3(4), Table 2 | Hydrophobic |
Di-8-ANEPPS | 940 | DRBIO, Cornell University | Online | Voltage-sensitive |
Dronpa-3 | 920 | Kao 2012 | no | GFP-like, reversibly switchable |
dsRed | 1000+ | DRBIO, Cornell University | Online | |
dsRed2 | 700-, 1050 | Drobizhev 2011 | Supp Figure 7 | |
EBFP2.0 | 750 | Drobizhev 2011 | Figure 1 | |
ECFP | 850 857 |
Spiess 2005 Drobizhev 2011 |
Figure 1 Supp Figure 2 |
|
EGFP | 927 | Drobizhev 2011 | Supp Figure 5 | |
EYFP | 970 | Spiess 2005 | Figure 1 | |
ER-Tracker white/blue | 728 | Bestvater 2002 | Figure 5 | Organelle tracker |
Evans Blue | 850 | Bennewitz 2014 | no | |
Fluo-3 | 800-810 > 940 | Mütze 2012 | Figure 4 | Calcium indicator |
Fluo-4 | 800 < 930 | Mütze 2012 | Figure 4 | Calcium indicator |
Fluo-4FF | 810 | Yasuda 2004 | No | Calcium indicator |
Fluo-5F | 810 | Yasuda 2004 | No | Calcium indicator |
Fluo-8 | 770-800, 940-1000 | Mütze 2012 | Figure 4 | Calcium indicator |
Fluorescein | 770-790 > 920 | DRBIO, Cornell University | Online | |
Fluorescein isiothiocyanate (FITC) | 800 | Wang 2012 | Figure 3 | |
Fura-2 (-Ca) | 730 | DRBIO, Cornell University | Online | Calcium indicator |
Fura-2 (+Ca) | 700 | DRBIO, Cornell University | Online | Calcium indicator |
GCaMP2 | 880-950 | Mütze 2012 | Figure 4 | Calcium indicator |
GCaMP3 | 940-1000 | Helassa 2015 | Figure 4I | Calcium indicator |
GCaMP3bright | 940-1000 | Helassa 2015 | Figure 4I | Calcium indicator |
GCaMP3fast | 940-1000 | Helassa 2015 | Figure 4H | Calcium indicator |
GCaMP6f | 940 | Harris lab, Janelia | Online | Calcium indicator |
GCaMP6s | 940 | Dana 2016 | Figure 5A | Calcium indicator |
GFP, wtGFP | 800 | DRBIO, Cornell University | Online | |
Hoechst 33258 | 560 | Trägårdh 2015 | Figure 5 | Nucleic acids |
Hoechst 33342 | 660 > 715 | Bestvater 2002 | Figure 3 | Nucleic acids |
Indo-1 (-Ca) | 690-, 730 | DRBIO, Cornell University | Online | Calcium indicator |
Indo-1 (+Ca) | 690- | DRBIO, Cornell University | Online | Calcium indicator |
jRCaMP1a | 1050-1150 | Dana 2016 | Figure 5A | Calcium indicator, OPO-friendly |
jRGECO1a | 1050-1150 | Dana 2016 | Figure 5A | Calcium indicator, OPO-friendly |
Katushka | 1080 | Drobizhev 2011 | Supp Figure 10 | |
Katushka2 | 1140 | Drobizhev 2011 | Supp Figure 8 | OPO-friendly |
Lissamine rhodamine | 837 >> 1116 | Bestvater 2002 | Figure 4 | |
Lucifer Yellow | 840 | DRBIO, Cornell University | Online | |
LysoTracker Red | 1010 < 1100 | Bestvater 2002 | Figure 5 | Organelle tracker |
LysoTracker Yellow | 972 | Bestvater 2002 | Figure 5 | Organelle tracker |
mAmetrine | 809 | Drobizhev 2011 | Supp Figure 5 | |
mBanana | 1070 | Drobizhev 2011 | Supp Figure 6 | OPO-friendly |
mCardinal | 1080+ | Chu 2014 | Supp Figure 3 | OPO-friendly |
mCerulean | 840 | Drobizhev 2011 | Supp Figure 2 | |
mCFP | 840 | DRBIO, Cornell University | Online | |
mCherry | 1070 < 1160 | Vadakkan 2009 | Figure 2 and 8 | OPO-friendly |
mCitrine | 950 | Drobizhev 2011 | Supp Figure 5 | |
mEGFP | 960 | DRBIO, Cornell University | Online | |
MitoTracker Red | 1133 | Bestvater 2002 | Figure 5 | Organelle tracker |
mKate | 1118 | Drobizhev 2011 | Supp Figure 9 | OPO-friendly |
mKate2 | 1140 | Drobizhev 2011 | Supp Figure 9 | OPO-friendly |
MNAH | 820 | Liu 2016 | no | Imaging of mitochondrial singlet oxygen during phototherapy |
mNeptune | 1104 | Drobizhev 2011 | Supp Figure 12 | OPO-friendly |
mNeptune1 | 1080+ | Chu 2014 | Supp Figure 3 | OPO-friendly |
mOrange | 1040-1100 | Drobizhev 2011 | Supp Figure 6 | OPO-friendly |
mRaspberry | 1118 | Drobizhev 2011 | Supp Figure 10 | OPO-friendly |
mRFP | 1030 | Svoboda 2006 | no (cf. Table 1) | |
mStrawberry | 1060 | Drobizhev 2011 | Supp Figure 8 | |
mVenus | 960 | DRBIO, Cornell University | Online | |
mWasabi | 927 | Drobizhev 2011 | Supp Figure 4 | |
NADH | 960 | DRBIO, Cornell University | Online | |
Near-infra Red Fluorescent Protein (iRFP) | 1260 | Filonov 2011 | Supp Figure 5 | OPO-friendly |
Pacific Blue | 780 | Lukomska 2006 | no | |
PerCP (Peridinin chlorophyll) | 850 | Bok 2015 | no | |
PhenGreen-FL | 1074- | Bestvater 2002 | Figure 3 | |
Phycoerythrin (PE) | 1064 | Chen 1997 | ? | |
PKH26 | 950 | Takaki 2015 | no | Membrane labelling |
PKH67 | 870 850 |
Morelli 2003 Kuwashima 2005 |
no | Membrane labelling |
Propidium iodide (PI) | 989 > 1015 >> 1099 | Bestvater 2002 | Figure 3 | Nucleic acids (dead cells) |
QD550 (organic) | broad (700-1000) | Larson 2003 | Figure 1A | Ultrabright |
QD550 (water-soluble) | broad (700-1000) | Larson 2003 | Figure 1A | Ultrabright |
QD567 (water-soluble) | broad (700-1000) | Larson 2003 | Figure 1A | Ultrabright |
QD605 (water-soluble) | broad (700-1000) | Larson 2003 | Figure 1A | Ultrabright |
QD630 (organic) | broad (700-1000) | Larson 2003 | Figure 1A | Ultrabright |
QuasAr1 | 1200 | Brinks 2015 | Table 1 | Voltage-sensitive |
QuasAr2 | 1200 | Brinks 2015 | Table 1 | Voltage-sensitive |
Quinacrine | 678 > 697 | Bestvater 2002 | Figure 3 | Nucleic acids |
RCaMP1h | 1070 | Harris lab, Janelia | Online | Calcium indicator |
Rhodamine 123 | 913 > 1090 | Bestvater 2002 | Figure 3 | |
Rhodamine B | 700 > 830 | DRBIO, Cornell University | Online | |
Rhodamine Green | 830-860 | Heinze 2000 | Figure 3 | |
Sapphire | 760-810 | DRBIO, Cornell University | Online | |
SeTa-632 | 760-980 | Podgorski 2012 | Figure 1 | Broad absorption spectrum |
SeTa-646 | 760-980 | Podgorski 2012 | Figure 1 | Broad absorption spectrum |
SeTa-670 | 760-980 | Podgorski 2012 | Figure 1 | Broad absorption spectrum |
SeTa-700 | 760-980 | Podgorski 2012 | Figure 1 | Broad absorption spectrum |
SeTau-647 | 760-980 | Podgorski 2012 | Figure 1 | Broad absorption spectrum |
SeTau-665 | 760-980 | Podgorski 2012 | Figure 1 | Broad absorption spectrum |
SNARF-1 | 837- | Bestvater 2002 | Figure 3 | pH indicator |
Sulforhodamine 101 | 870-920 | Appaix 2012 | Figure 2A | |
Sulforhodamine B | 800-850 | Appaix 2012 | Figure 2A | |
SybrGreen | 790, 870-900 | Neu 2002 | Table 1 | Nucleic acids |
SybrGold | 760, 850-890 | Neu 2002 | Table 1 | Nucleic acids |
Syto 9 | 860-890 | Neu 2002 | Table 1 | Nucleic acids |
Syto 13 | 860-890 | Neu 2002 | Table 1 | Nucleic acids |
Syto 40 | 830-860 | Neu 2002 | Table 1 | Nucleic acids |
Sytox Blue | 840-870 | Neu 2002 | Table 1 | Nucleic acids |
Sytox Green | 630- > 715 | Trägårdh 2015 | Figure 5 | Nucleic acids |
TagRFP | 759 > 1050 | Drobizhev 2011 | Supp Figure 7 | |
tdRFP | 1110 | Herz 2010 | Figure 2B | OPO-friendly |
tdTomato | 1060 | Drobizhev 2011 | Supp Figure 7 | OPO-friendly |
tdKatushka2 | 1100 | Drobizhev 2011 | Supp Figure 9 | OPO-friendly |
Tetramethylrhodamine (TRITC) | 840 | Wang 2012 | Figure 3 | |
Texas Red | 760 - 810 | Heinze 2000 | Figure 3 | |
Thiophene DiHemiCyanine (TDHC) | 740 | Baek 2016 | Figure 5a | Viscosity-sensitive dye |
TO-PRO-3 | 1110 | Smith 2012 | no | Nucleic acids, OPO-friendly |
VivoTag 680 | 820 | Swirski 2007 | no | |
YFP | 960 | DRBIO, Cornell University | Online |