Two-photon excitation fluorescence suitable dyes


This page provides a list of two-photon probes as well as a matching wavelength range that has been already used,
and the reference where the two-photon absorption spectrum can be found.



Number of two-photon probes:

280

Last update: May 11th, 2018 (review paper published in Brain Structure and Function)



I recently wrote a review paper compiling the two-photon spectroscopic properties of 280+ fluorescent probes.
The view-only paper is freely available here: https://rdcu.be/N2tv

Don't forget to share it and cite it in your papers ;)

Enter your email below to get a pdf of the paper within a few days.






(Note: the list below is outdated, I recommend you use the paper instead)
Name Wavelength(s) for two-photon excitation Reference or URL Available spectrum Comments
Acridine Orange 837 > 882 >> 981 Bestvater 2002 Figure 3 Nucleic acids
Alexa Fluor 430 910 (880-940) Mütze 2012 Figure 2
Alexa Fluor 488 720-800, 900-1000 Mütze 2012 Figure 2
Alexa Fluor 514 750-800, 950-1000 Mütze 2012 Figure 2
Alexa Fluor 546 720-840, 950-1000 Mütze 2012 Figure 2
Alexa Fluor 568 720-840 Mütze 2012 Figure 2
Alexa Fluor 594 720-850 Mütze 2012 Figure 2
Alexa Fluor 610 720-900 Mütze 2012 Figure 2
Alexa Fluor 633 720-900 Mütze 2012 Figure 2 Artery labelling (see Shen 2012)
Alexa Fluor 647 1240 Kobat 2009 Figure 7 (far-red) OPO-friendly
Alexa Fluor 680 1280 Kobat 2009 Figure 7 (far-red) OPO-friendly
Alexa Fluor 700 1300 Kobat 2009 Figure 7 (far-red) OPO-friendly
Aminomethylcoumarin (AMC) 703 < 722 >> 863 Bestvater 2002 Figure 3
Aminomethyl coumarin acetate (AMCA) 700 Bok 2015 no
Ant2-PHEA 800 Mettra 2016 Figure 6 Endothelium staining
ArcLight A242 950 Brinks 2015 Table 1 Voltage-sensitive
ASAP1 950 Brinks 2015 Table 1 Voltage-sensitive
Bodipy (in water) 920 DRBIO, Cornell University Online Calcium indicator
Bodipy FL (in DMSO) 920 << 972 Bestvater 2002 Figure 3
Bodipy TRoad 1032 << 1108 Bestvater 2002 Figure 3
Brilliant Violet 421 (BV421) 710-1000 Chattopadhyay 2012 no (cf. Fig 6G)
CAESR 968 Brinks 2015 Table 1 Voltage-sensitive
Cal-590 1050 Tischbirek 2015 Figure 1B Calcium indicator
Calcium Crimson 850-920 DRBIO, Cornell University Online Calcium indicator
Calcium Green 950 > 820 DRBIO, Cornell University Online Calcium indicator
Calcium Orange 820 DRBIO, Cornell University Online Calcium indicator
Calcofluor White 590 Trägårdh 2015 Figure 5 Cellulose and chitin binding
CaRuby-Cl 912 Collot 2012 Figure 5 Calcium indicator
CaRuby-F 917 Collot 2012 Figure 5 Calcium indicator
CaRuby-Me 917 Collot 2012 Figure 5 Calcium indicator
Cascade Blue 700-800 DRBIO, Cornell University Online ! Poorly efficient
CellTracker Blue (CMAC) 780 Zinselmeyer 2009 no
CellTracker Orange (CMTMR) 820 Miller 2002 no
CFP 840 Zipfel 2003 Figure 3b
CFSE 780 Miller 2002 no
Citrine 968 Drobizhev 2011 Figure 1
Coumarin 307 800 DRBIO, Cornell University Online
Cy2 837 < 908 > 981 Bestvater 2002 Figure 4
Cy3 1032 Bestvater 2002 Figure 4
Cy5.5 1280 Kobat 2009 Figure 7 (far-red) OPO-friendly
DAPI 580 >> 685 Trägårdh 2015 Figure 2 Nucleic acids
DCF2 (H2DCFDA) (in DMSO) 1065 Bestvater 2002 no
DiA 880 Ruthazer 2002 Table 1 Lipophilic Tracer
DiD 830 Ruthazer 2002 Table 1 Lipophilic Tracer
DiI 750-, 1000-1050 DRBIO, Cornell University Online Lipophilic Tracer
DiO 880 Ruthazer 2002 Table 1 Lipophilic Tracer
Di-4-ANEPPS 940 DRBIO, Cornell University Online Voltage-sensitive
Di-8-ANEPPDHQ 900-950 Fisher 2015 Figure 17.3 Voltage-sensitive
Di-stryl benzene derivative:
2,5-Dicyano-1,4-bis[2-(4-(di-n-butylamino)phenyl)Vinyl]benzene		 830 Pond 2002 Figure 3(1), Table 2 Hydrophobic
Di-stryl benzene derivative:
2,5-Dicyano-1,4-bis[2-(4-(diphenylamino)phenyl)Vinyl]benzene		 830 Pond 2002 Figure 3(2), Table 2 Hydrophobic
Di-stryl benzene derivative:
1,4-Bis[1-cyano-2-(4-(di-n-butylamino)phenyl)Vinyl]benzene		 790 Pond 2002 Figure 3(3), Table 2 Hydrophobic
Di-stryl benzene derivative:
1,4-Bis[1-cyano-2-(4-(diphenylamino)phenyl)Vinyl]benzene		 825 Pond 2002 Figure 3(4), Table 2 Hydrophobic
Di-8-ANEPPS 940 DRBIO, Cornell University Online Voltage-sensitive
Dronpa-3 920 Kao 2012 no GFP-like, reversibly switchable
dsRed 1000+ DRBIO, Cornell University Online
dsRed2 700-, 1050 Drobizhev 2011 Supp Figure 7
EBFP2.0 750 Drobizhev 2011 Figure 1
ECFP 850
857		 Spiess 2005
Drobizhev 2011		 Figure 1
Supp Figure 2
EGFP 927 Drobizhev 2011 Supp Figure 5
EYFP 970 Spiess 2005 Figure 1
ER-Tracker white/blue 728 Bestvater 2002 Figure 5 Organelle tracker
Evans Blue 850 Bennewitz 2014 no
Fluo-3 800-810 > 940 Mütze 2012 Figure 4 Calcium indicator
Fluo-4 800 < 930 Mütze 2012 Figure 4 Calcium indicator
Fluo-4FF 810 Yasuda 2004 No Calcium indicator
Fluo-5F 810 Yasuda 2004 No Calcium indicator
Fluo-8 770-800, 940-1000 Mütze 2012 Figure 4 Calcium indicator
Fluorescein 770-790 > 920 DRBIO, Cornell University Online
Fluorescein isiothiocyanate (FITC) 800 Wang 2012 Figure 3
Fura-2 (-Ca) 730 DRBIO, Cornell University Online Calcium indicator
Fura-2 (+Ca) 700 DRBIO, Cornell University Online Calcium indicator
GCaMP2 880-950 Mütze 2012 Figure 4 Calcium indicator
GCaMP3 940-1000 Helassa 2015 Figure 4I Calcium indicator
GCaMP3bright 940-1000 Helassa 2015 Figure 4I Calcium indicator
GCaMP3fast 940-1000 Helassa 2015 Figure 4H Calcium indicator
GCaMP6f 940 Harris lab, Janelia Online Calcium indicator
GCaMP6s 940 Dana 2016 Figure 5A Calcium indicator
GFP, wtGFP 800 DRBIO, Cornell University Online
Hoechst 33258 560 Trägårdh 2015 Figure 5 Nucleic acids
Hoechst 33342 660 > 715 Bestvater 2002 Figure 3 Nucleic acids
Indo-1 (-Ca) 690-, 730 DRBIO, Cornell University Online Calcium indicator
Indo-1 (+Ca) 690- DRBIO, Cornell University Online Calcium indicator
jRCaMP1a 1050-1150 Dana 2016 Figure 5A Calcium indicator, OPO-friendly
jRGECO1a 1050-1150 Dana 2016 Figure 5A Calcium indicator, OPO-friendly
Katushka 1080 Drobizhev 2011 Supp Figure 10
Katushka2 1140 Drobizhev 2011 Supp Figure 8 OPO-friendly
Lissamine rhodamine 837 >> 1116 Bestvater 2002 Figure 4
Lucifer Yellow 840 DRBIO, Cornell University Online
LysoTracker Red 1010 < 1100 Bestvater 2002 Figure 5 Organelle tracker
LysoTracker Yellow 972 Bestvater 2002 Figure 5 Organelle tracker
mAmetrine 809 Drobizhev 2011 Supp Figure 5
mBanana 1070 Drobizhev 2011 Supp Figure 6 OPO-friendly
mCardinal 1080+ Chu 2014 Supp Figure 3 OPO-friendly
mCerulean 840 Drobizhev 2011 Supp Figure 2
mCFP 840 DRBIO, Cornell University Online
mCherry 1070 < 1160 Vadakkan 2009 Figure 2 and 8 OPO-friendly
mCitrine 950 Drobizhev 2011 Supp Figure 5
mEGFP 960 DRBIO, Cornell University Online
MitoTracker Red 1133 Bestvater 2002 Figure 5 Organelle tracker
mKate 1118 Drobizhev 2011 Supp Figure 9 OPO-friendly
mKate2 1140 Drobizhev 2011 Supp Figure 9 OPO-friendly
MNAH 820 Liu 2016 no Imaging of mitochondrial singlet oxygen
during phototherapy
mNeptune 1104 Drobizhev 2011 Supp Figure 12 OPO-friendly
mNeptune1 1080+ Chu 2014 Supp Figure 3 OPO-friendly
mOrange 1040-1100 Drobizhev 2011 Supp Figure 6 OPO-friendly
mRaspberry 1118 Drobizhev 2011 Supp Figure 10 OPO-friendly
mRFP 1030 Svoboda 2006 no (cf. Table 1)
mStrawberry 1060 Drobizhev 2011 Supp Figure 8
mVenus 960 DRBIO, Cornell University Online
mWasabi 927 Drobizhev 2011 Supp Figure 4
NADH 960 DRBIO, Cornell University Online
Near-infra Red Fluorescent Protein (iRFP) 1260 Filonov 2011 Supp Figure 5 OPO-friendly
Pacific Blue 780 Lukomska 2006 no
PerCP (Peridinin chlorophyll) 850 Bok 2015 no
PhenGreen-FL 1074- Bestvater 2002 Figure 3
Phycoerythrin (PE) 1064 Chen 1997 ?
PKH26 950 Takaki 2015 no Membrane labelling
PKH67 870
850		 Morelli 2003
Kuwashima 2005		 no Membrane labelling
Propidium iodide (PI) 989 > 1015 >> 1099 Bestvater 2002 Figure 3 Nucleic acids (dead cells)
QD550 (organic) broad (700-1000) Larson 2003 Figure 1A Ultrabright
QD550 (water-soluble) broad (700-1000) Larson 2003 Figure 1A Ultrabright
QD567 (water-soluble) broad (700-1000) Larson 2003 Figure 1A Ultrabright
QD605 (water-soluble) broad (700-1000) Larson 2003 Figure 1A Ultrabright
QD630 (organic) broad (700-1000) Larson 2003 Figure 1A Ultrabright
QuasAr1 1200 Brinks 2015 Table 1 Voltage-sensitive
QuasAr2 1200 Brinks 2015 Table 1 Voltage-sensitive
Quinacrine 678 > 697 Bestvater 2002 Figure 3 Nucleic acids
RCaMP1h 1070 Harris lab, Janelia Online Calcium indicator
Rhodamine 123 913 > 1090 Bestvater 2002 Figure 3
Rhodamine B 700 > 830 DRBIO, Cornell University Online
Rhodamine Green 830-860 Heinze 2000 Figure 3
Sapphire 760-810 DRBIO, Cornell University Online
SeTa-632 760-980 Podgorski 2012 Figure 1 Broad absorption spectrum
SeTa-646 760-980 Podgorski 2012 Figure 1 Broad absorption spectrum
SeTa-670 760-980 Podgorski 2012 Figure 1 Broad absorption spectrum
SeTa-700 760-980 Podgorski 2012 Figure 1 Broad absorption spectrum
SeTau-647 760-980 Podgorski 2012 Figure 1 Broad absorption spectrum
SeTau-665 760-980 Podgorski 2012 Figure 1 Broad absorption spectrum
SNARF-1 837- Bestvater 2002 Figure 3 pH indicator
Sulforhodamine 101 870-920 Appaix 2012 Figure 2A
Sulforhodamine B 800-850 Appaix 2012 Figure 2A
SybrGreen 790, 870-900 Neu 2002 Table 1 Nucleic acids
SybrGold 760, 850-890 Neu 2002 Table 1 Nucleic acids
Syto 9 860-890 Neu 2002 Table 1 Nucleic acids
Syto 13 860-890 Neu 2002 Table 1 Nucleic acids
Syto 40 830-860 Neu 2002 Table 1 Nucleic acids
Sytox Blue 840-870 Neu 2002 Table 1 Nucleic acids
Sytox Green 630- > 715 Trägårdh 2015 Figure 5 Nucleic acids
TagRFP 759 > 1050 Drobizhev 2011 Supp Figure 7
tdRFP 1110 Herz 2010 Figure 2B OPO-friendly
tdTomato 1060 Drobizhev 2011 Supp Figure 7 OPO-friendly
tdKatushka2 1100 Drobizhev 2011 Supp Figure 9 OPO-friendly
Tetramethylrhodamine (TRITC) 840 Wang 2012 Figure 3
Texas Red 760 - 810 Heinze 2000 Figure 3
Thiophene DiHemiCyanine (TDHC) 740 Baek 2016 Figure 5a Viscosity-sensitive dye
TO-PRO-3 1110 Smith 2012 no Nucleic acids, OPO-friendly
VivoTag 680 820 Swirski 2007 no
YFP 960 DRBIO, Cornell University Online


(c) Daniel Fiole 2018